Ligand Binding Assays

The methods applied in our lab are used to inves­ti­gate binding inter­ac­tions of mole­cules. Resulting para­me­ters include kinetics, affinity, speci­ficity, concen­tra­tion and content. We have broad expe­ri­ence in providing tailor-made appli­ca­tions to help under­stand binding inter­ac­tions in detail.

We offer setup of methods based on quality by design (QbD) prin­ci­ples. Estab­lished proto­cols are avail­able for a wide selec­tion of protein inter­ac­tions (such as Fc receptor binding and C1q binding) and may be adapted to your specific needs. Method qual­i­fi­ca­tion and vali­da­tion is performed in accor­dance with the applic­able guide­lines, e.g. ICH Q2R1.

Surface Plasmon Reso­nance (SPR, Biacore T200)

  • Biomol­e­c­ular inter­ac­tion analysis using a real-time and label-free system

Binding kinetics and affinity

Reportable values:

  • Asso­ci­a­tion rate constant ka (M‑1 s‑1)
  • Disso­ci­a­tion rate constant kd (s‑1)
  • Equi­lib­rium constant KD (M)

Sensor­gram comparison

Reportable value:

  • Simi­larity score (%)
  • Compar­ison of samples to pool” of refer­ence data -> simi­larity corridor
  • Applic­able for complex inter­ac­tions without defined fitting model

Surro­gate potency assays

Reportable value:

  • % rela­tive potency compared to reference
  • Dose-response curve
  • Eval­u­a­tion: PLA, 4PL

Cali­bra­tion-free concen­tra­tion analysis (CFCA)

Reportable value: active concentration

  • Estab­lished assays, e.g.: Fc receptor kinetics and affinity: FcRn, FcγRIa, FcγRIIa, FcγRIIb, FcγRIIIa, FcγRIIIb

ELISA

  • different assay formats, such as direct, indi­rect, sand­wich or compet­i­tive ELISA can be applied
  • Deter­mi­na­tion of rela­tive potency
  • Concen­tra­tion / content of analyte

Estab­lished assays, e.g.: C1q binding (potency), Residual host cell protein (HCP) determination

Glyco­sy­la­tion analysis

  • Lectin array tech­nology (Glyco­Sta­tion)
We Value Your Privacy
We use cookies on our website. Some of them are essential, while others help us to analyze how this website is being used and to allow you to contact us through our website, i.e. use the chat widget. You can change your decision at any time.
We Value Your Privacy
Statistics
We use these technologies to analyze how this website is being used.
Name Google Analytics, Google Tag Manager
Provider Google Ireland Limited, Gordon House, Barrow Street, Dublin 4, Ireland
Purpose Cookie by Google used for website analytics. Generates statistical data on how the visitor uses the website.
Privacy Policy https://policies.google.com/privacy
Cookie Name _ga, _gat, _gid
Cookie Expiry 2 years

 

Name LinkedIn Corporation
Provider LinkedIn Corporation, 605 W Maude Ave, Sunnyvale, CA 94085, USA
Purpose Cookie by LinkedIn used for website analytics. Generates statistical data on how the visitor uses the website.
Privacy Policy https://www.linkedin.com/legal/privacy-policy
Cookie Name
Cookie Expiry 2 years
Customer Interaction
These technologies will allow you to contact us through our website, i.e. use the chat widget.
Name LiveChat
Provider LiveChat Software S.A., ul. Zwycięska 47, 53-033 Wroclaw, Poland
Purpose Communication with clients via online chat using the API of the chat service LiveChat.
Privacy Policy https://www.livechat.com/legal/privacy-policy/
Cookie Name __lc_cid, __lc_cst
Cookie Expiry 2 years
Essential
Technologies required to enable the core functionality of this website.
Name Cookie Consent
Provider Owner of this website, Imprint
Purpose Saves the visitors preferences selected in the cookie banner.
Cookie Name ws_cookie_consent
Cookie Expiry 1 year