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Ligand Binding Assays
The methods applied in our lab are used to investigate binding interactions of molecules. Resulting parameters include kinetics, affinity, specificity, concentration and content. We have broad experience in providing tailor-made applications to help understand binding interactions in detail. We offer setup of methods based on quality by design (QbD) principles. Established protocols are available for a wide selection of proteininteractions (such as Fc receptor binding and C1q binding) and may be adapted to your specific needs. Method qualification and validation is performed in accordance with the applicable guidelines, e.g. ICH Q2R1.
Surface Plasmon Resonance (SPR, Biacore T200)
- Biomolecular interaction analysis using a real-time and label-free system
Binding kinetics and affinity
Reportable values:
- Association rate constant ka (M-1 s-1)
- Dissociation rate constant kd (s-1)
- Equilibrium constant KD (M)
Sensorgram comparison
Reportable value:
- Similarity score (%)
- Comparison of samples to „pool” of reference data -> similarity corridor
- Applicable for complex interactions without defined fitting model
Surrogate potency assays
Reportable value:
- % relative potency compared to reference
- Dose-response curve
- Evaluation: PLA, 4PL
Calibration-free concentration analysis (CFCA)
Reportable value: active concentration
- Established assays, e.g.: Fc receptor kinetics and affinity: FcRn, FcγRIa, FcγRIIa, FcγRIIb, FcγRIIIa,FcγRIIIb
ELISA – different assay formats, such as direct, indirect, sandwich or competitive ELISA can beapplied.
- Determination of relative potency
- Concentration / content of analyte
Established assays, e.g.: C1q binding (potency), Residual host cell protein (HCP) determination
Glycosylation analysis
- Lectin array technology (GlycoStation)
CONTACT US – we are looking forward to supporting your projects!