Method overview - ELISA

The enzyme-linked immunosorbent assay (ELISA) is an analytical biochemistry assay, used to quantitatively or qualitatively analyze the presence of a specific substance (“analyte”) in a liquid sample. The base principle of this method is the interaction between antibody and the respective antigen.

In general, the analyte of interest is either coated onto a surface or covalently bound. Subsequently an enzyme linked antibody (“detection antibody”) is applied and binds to the antigen. In the final step, the substrate of the enzyme is added to the plate. The detected signal (e.g. a change in color or a fluorescence signal) is proportional to the amount of the analyte in the sample.