Cellbased Assays I VelaLabs

Host cell protein residue ELISA

The detection of potential Host Cell Protein (HCP) impurities from HEK 293 cells are detected and quantified using the Cygnus HEK 293 Host Cell Proteins Kit. The method is based on a two-site immunoenzymatic assay using an HRP-labelled anti-HEK antibody to detect cell residues. The samples are applied on anti-HEK antibody coated microtiter stripes forming a complex. After removal of unbound samples, 3,3,5,5-Tetramethylbenzidine (TMB) is added to the stripe and converted by the HRP, resulting in a color signal that is directly proportional to the concentration of the HEK HCPs present in the sample. In the final step, this reaction is stopped by acidification and the optical density (OD) of each well is measured using the ELISA microplate reader SpectraMax.

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