Cellbased Assays I VelaLabs

PD-1/PD-L1 Competition ELISA

The principle of the PD-1/PD-L1 competition assay is an indirect ELISA in which soluble PD-1 is coated onto the surface of a 96-well plate. Next, the samples are incubated simultaneously with a constant concentration of biotinylated PD-L1, both competing for binding to PD-1. HRP-labelled streptavidin is subsequently binding to the biotinylated PD-L1 but not the unlabeled sample. 3,3,5,5-Tetramethylbenzidine (TMB) is converted by the HRP, resulting in a color signal that is inverse proportional to the concentration of the sample. In the final step, this reaction is stopped by acidification and the optical density (OD) of each well is measured using the ELISA microplate reader SpectraMax.

Logo
We Value Your Privacy
We use cookies on our website. Some of them are essential, while others help us to analyze how this website is being used and to allow you to contact us through our website, i.e. use the chat widget. You can change your decision at any time.
We Value Your Privacy
Statistics
We use these technologies to analyze how this website is being used.
Name Google Analytics, Google Tag Manager
Provider Google Ireland Limited, Gordon House, Barrow Street, Dublin 4, Ireland
Purpose Cookie by Google used for website analytics. Generates statistical data on how the visitor uses the website.
Privacy Policy https://policies.google.com/privacy
Cookie Name _ga, _gat, _gid
Cookie Expiry 2 years

 

Name LinkedIn Corporation
Provider LinkedIn Corporation, 605 W Maude Ave, Sunnyvale, CA 94085, USA
Purpose Cookie by LinkedIn used for website analytics. Generates statistical data on how the visitor uses the website.
Privacy Policy https://www.linkedin.com/legal/privacy-policy
Cookie Name
Cookie Expiry 2 years
Customer Interaction
These technologies will allow you to contact us through our website, i.e. use the chat widget.
Name LiveChat
Provider LiveChat Software S.A., ul. Zwycięska 47, 53-033 Wroclaw, Poland
Purpose Communication with clients via online chat using the API of the chat service LiveChat.
Privacy Policy https://www.livechat.com/legal/privacy-policy/
Cookie Name __lc_cid, __lc_cst
Cookie Expiry 2 years
Essential
Technologies required to enable the core functionality of this website.
Name Cookie Consent
Provider Owner of this website, Imprint
Purpose Saves the visitors preferences selected in the cookie banner.
Cookie Name ws_cookie_consent
Cookie Expiry 1 year